METHODS FOR DETECTING METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS AND PHENOTYPIC CHARACTERIZATION OF STAPHYLOCOCCI

Abstract

The researchers wanted to look at MRSA and MSSA antibiotic susceptibility patterns, as well as community-acquired MRSA and hospital-acquired MRSA, and coagulase-negative staphylococci. Sputum, blood, pus, urine, and bodily fluids, among other clinical samples, were used to isolate MRSA bacteria. During the examination, 600 samples were cultured positive for bacteria. MRSA isolates were detected using the disc diffusion methods of oxacillin (1????g) and cefoxitin (30????g). 108 (43.02%) of the 210 Staphylococcus aureus isolates were classified as MRSA according to cefoxitin resistance, while 102 (40.63%) were classified as MSSA due to cefoxitin susceptibility. Hospital-acquired MRSA made up 82% of the total, whereas community-acquired MRSA made up 18%. Patients under the age of 40 years were significantly more impacted by Methicillin-Resistant Staphylococcus aureus than those in other age groups in terms of age and sex distribution of Staphylococcus aureus. Cefoxitin disc diffusion had a higher sensitivity and specificity for detecting MRSA than other phenotypic approaches. Cefoxitin, commonly known as cephamycin, is more potent than penicillin at inducing the mecA regulatory system.